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Instructions Protocol may be improved. Intra-assay Precision Precision within an assay: Three samples of known concentration were tested twenty times on one plate to assess. To assess the linearity of the assay, samples were spiked with high concentrations of human 25HVD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

The recovery of human 25HVD3 spiked to levels throughout the range of the assay in various matrices was evaluated.


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