Aggressive post-World War II growth and expansion of El Paso into the surrounding desert, as is common in the US Southwest, placed newer housing onto more pristine land surfaces. This is reflected in generally low-to-background levels of heavy metals in these newer areas of the city. Thus there is a strong contamination and heavy metals exposure risk gradient between older and newer neighborhoods within a single city. Una segunda etapa estuvo orientada a determinar la estructura sexo-etaria de la muestra. Analyses were carried out in different stages. First, a detailed inventory of the collection was made and some taphonomic variables were considered in order to evaluate the general condition of the bone remains.
A second phase was oriented to the determination of the age-sex structure of the sample. Finally, occupational stress markers studies were undertaken along with the observation of certain infectious and traumatic pathologies. As a whole, these analyses show the. This study analyzed what effect commuters had on wage levels, employment, and industrial locations, and the relative importance of the commuter as part of the work force.
A questionnaire was administered to workers and to their 20 employers in the apparel industry of El Paso , Texas. The questionnaires revealed that the commuters were paid the…. Al volver la esquina de Carmen Laforet. The TU Berlin has developed a family of planetary rovers for educational use and research activities.
The paper will introduce these cost-effective systems, which can be used for analogue mission demonstration on Earth. Sequence analysis indicated that P. In this Letter, a generalized Tu formula is firstly presented to construct Hamiltonian structures of fractional soliton equations. The obtained results can be reduced to the classical Hamiltonian hierarchy of AKNS in ordinary calculus. TU Dresden operates an accelerator-based intensive DT neutron generator. The neutron source is a solid-type water-cooled tritium target based on a titanium matrix on a copper carrier.
A pneumatic sample transport system is available for short-time irradiations and connected to wo high-purity germanium detector spectrometers for the measurement of induced activities. The overall design of the experimental hall with the neutron generator allows a flexible setup of experiments including the possibility of investigating larger structures and cooled samples or samples at high temperatures.
Full Text Available Alfonso Paso was, without doubt, the most prolific of the post-war Spanish playwrights. His numerous theatrical productions, of which there are more than a hundred comedies, led the playwright to a popularity which in turn permitted him to rise within the theatrical hegemony of the time, filling the Spanish stage with an unending series of plays with clear commercial nuances in which the main element is humour. This humour was conceived for the humoristic sensibility of the growing middle class at the end of the fifties and the beginning of the sixties, whose tastes and interests were ideal material for Paso.
This article is written with this in mind, and various aspects present in the works of Alfonso Paso are analysed, such as the middle class, the role of women, social changes, and the influence of tourism and immigration in the Spain of that period. A neutron scattering study of the ternary complex EF- Tu. Changes in the microbiota may influence medication efficacy and interactions. Daikenchuto TU , a herbal medication, comprised of ginger, ginseng, and Japanese pepper, is widely used in Japanese traditional Kampo medicine for intestinal motility and postoperative paralytic ileus.
We previously showed in mice that consumption of TU for 4 weeks changed the gut microbiota and increased bioavailability of bacterial ginsenoside metabolites. Since TU is prescribed in humans for months to years, we examined the time- and sex-dependent effects of TU on mouse gut microbiota. Oral administration of 1. Changes in both sexes largely reverted to baseline upon TU withdrawal. Effects were time and dose dependent. The microbial profiles reverted to baseline within 4 weeks after withdrawal of 0.
In summary, dietary TU changed mouse microbiota in a time-, sex-, and dose-dependent manner. These findings may be taken into consideration when determining optimizing dose for conditions of human health and disease with the consideration of differences in composition and response of the human intestinal microbiota. Aerosol characterizaton in El Paso -Juarez airshed using optical methods. The assessment and characterization of atmospheric aerosols and their optical properties are of great significance for several applications such as air pollution studies, atmospheric visibility, remote sensing of the atmosphere, and impacts on climate change.
Decades ago, the interest in atmospheric aerosols was primarily for visibility impairment problems; however, recently interest has intensified with efforts to quantify the optical properties of aerosols, especially because of the uncertainties surrounding the role of aerosols in climate change. The main objective of the optical characterization of aerosols is to understand their properties. These properties are determined by the aerosols' chemical composition, size, shape and concentration.
The general purpose of this research was to contribute to a better characterization of the aerosols present in the Paso del Norte Basin. This study permits an alternative approach in the understanding of air pollution for this zone by analyzing the predominant components and their contributions to the local environment. This dissertation work had three primary objectives, in which all three are intertwined by the general purpose of the aerosol characterization in the Paso del Norte region.
The first objective was to retrieve the columnar aerosol size distribution for two different cases clean and polluted scenarios at each season spring, summer, fall and winter of the year The purpose of this objective was to provide an alternate method of quantifying and size-allocating aerosols in situ, by using the optical properties of the aerosols and inversely reconstruct and.
Incluso en los casos de poliposis, por ejemplo, rara vez se ve afectada la mucosa que recubre la convexidad nasolagrimal. Incluso en los casos de poliposis nasosinusal, rara vez se ve. Globalisation, maquiladoras and transnational identities at the US-Mexico border: Hitherto, studies on border identities have emphasized more socio-cultural processes and have not analysed economic processes sufficiently as decisive in the construction of identities.
Airborne-electromagnetic and earth-resistivity surveys were used to explore for fresh ground water in the Hueco Bolson southeast of El Paso , Texas. Aerial surveys were made along about miles km of flight line, and 67 resistivity soundings were made along miles km of profile. The surveys did not indicate the presence of any large bodies of fresh ground water, but several areas may be underlain by small to moderate amounts of fresh to slightly saline water.
The material underlying the flood plain of the Rio Grande is predominantly clay or sand of low resistivity. Along a band on the mesa next to and parallel to the flood plain, more resistive material composed partly of deposits of an ancient river channel extends to depths of about to 1, feet to m. Locally, the lower part of this more resistive material is saturated with fresh to slightly saline water. The largest body of fresh to slightly saline ground water detected in this study is between Fabens and Tornillo, Texas, mostly in the sandhill area between the flood plain and the mesa.
Under assumed conditions, the total amount of water in storage may be as much as , to , acre-feet million to 1 billion m. The resistivity data indicate that the deep artesian zone southwest of Fabens extends from a depth of about 1, feet m to about 2, feet m. Radiographic and pathologic features of osteopetrosis in two Peruvian Paso foals. The radiographic and pathologic findings of two Peruvian Paso foals with osteopetrosis are described. Both foals, one male and one female, presented with respiratory difficulty, brachygnathia and failure to rise after birth.
Both foals were mildly anemic, hypogammaglobulinemic and had elevations in serum alkaline phosphatase. Increased medullary bone opacity was noted on radiographs of the extremities, spine and skull in both foals.
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A lack of normal cortical: The medullary primary spongiosa appeared to run in parallel columns away from the physes of all long bones and the vertebrae. This created a distinctive hour glass appearance to the osteopetrotic bones. One foal developed a bacterial pneumonia. Both foals were euthanized due to failure to thrive. Histopathology and electron microscopy documented these foals to have normal osteoclastic numbers but lack normal ruffled borders, lack of a clear zone and normal lysosomal numbers indicative of cellular dysfunction. These clinical, radiographic and pathologic findings are similar to the juvenile, lethal autosomal recessive form of osteopetrosis described in humans.
Osteopetrosis has not been previously described in a female foal. El nacimiento hospitalario e intervencionista: De esta forma, se analiza una perspectiva compleja: Existen pocas organizaciones binacionales de mujeres, con algunas excepciones en los aspectos de salud y violencia. The purpose of the program was to assist the University in building the infrastructure required for its emerging role as a regional center for energy-related research.
The program had four major objectives, as follows: Each of the above objectives has been exceeded substantially, and, as a consequence, the University has become a regional leader in energy and environmental research and outreach efforts. This test will be done over a one month timeframe. The project will also analyze. Final inform of the studies carried out by the Geological Institute of Uruguay in the zone of Paso Severino of Santa Lucia small river. This inform is about the geological conditions study carried out in Paso Severino zone of the Santa Lucia small river to make a dam to provide water to Montevideo.
The techniques used were photo interpretation and general geological studies. Motores de paso , control de movimiento. This paper focuses on the position of stepping motors in current industrial applications, recent advances andgeneral trends in desi Bacterial translation elongation factor EF- Tu interacts and colocalizes with actin-like MreB protein.
We show that translation initiation factor EF- Tu plays a second important role in cell shape maintenance in the bacterium Bacillus subtilis. EF- Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal element setting up rod cell shape. Lowering of the EF- Tu levels had a minor effect on translation but a strong effect on cell shape and on the localization of MreB, and blocking of the function of EF- Tu in translation did not interfere with the localization of MreB, showing that, directly or indirectly, EF- Tu affects the cytoskeletal MreB structure and thus serves two important functions in a bacterium.
Full Text Available Abstract Background Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. Results Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvf TU mutant and in its B. In addition to the reduced plcR expression in the yvf TU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses.
The virulence of the yvf TU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. Conclusion The Yvf TU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. Full Text Available National Road no. It is crossed by a secondary road by means of an elevated crossing, which can carry light vehicles and pedestrians. The crossing is The width of the deck is 5. The structure is a boxgirder. It is a prestressed, continuous beam, which runs along a curve.
Access to the bridge is along ramps built on soil embankments. Owing to the curved planform of the crossing, the torsion forces on the structure are relatively important, but the special distributions of the prestressing cables has made it possible to counteract this loading effect in a satisfactory manner. The hollow reinforced concrete supports are trapezoidal in cross-section, and have hinges at the top and 1 bottom. Thus they act as pendules. The tubes containing the prestressing cables are injected with cement after the cables have been stretched, in accordance with the technique of the contractors, Messrs.
La anchura del tablero es de 5,5 m, de los que 4 m corresponden a la calzada y 1,50 a dos andenes laterales e iguales. Unauthorized border crossings and migrant deaths: We examined the major causes of and risk factors for death among migrants who died while making unauthorized border crossings into the United States from Mexico. Decedents were included in the study if 1 their remains were found between January 1, , and December 31, , in any US county along the mi km section of the US-Mexican border from Yuma, Ariz, to El Paso , Tex; 2 their immigration status was unauthorized; and 3 they were believed to have died during transit from Mexico to the United States.
Characteristics of the decedents and causes of and risk factors for their deaths were examined. Deaths among migrants making unauthorized crossings of the US-Mexican border are due to causes that are largely preventable. Prevention strategies should target young Mexican men, and focus on preventing them from conceiving plans to cross the border, discouraging them from using dangerous routes as crossing points, and providing search-and-rescue teams to locate lost or injured migrant crossers.
Unauthorized Border Crossings and Migrant Deaths: Sapkota, Sanjeeb; Kohl, Harold W. Decedents were included in the study if 1 their remains were found between January 1, , and December 31, , in any US county along the mi km section of the US—Mexican border from Yuma, Ariz, to El Paso , Tex; 2 their immigration status was unauthorized; and 3 they were believed to have died during transit from Mexico to the United States.
Deaths among migrants making unauthorized crossings of the US—Mexican border are due to causes that are largely preventable. To compare demographics and motivations for falls from bridges at the United States-Mexico border and in El Paso County, Texas, and to analyze injuries and injury patterns to support intentionality and to provide treatment recommendations.
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A retrospective observational review was conducted of hospital admissions to a trauma center after falls from bridges from to Statistical methods used were chi-square testing, T-test for means comparison, univariate correlations, and regression analysis. Of the 97 evaluated patients, Falls over the border were associated with more immigration-related motivations and fewer suicide attempts.
Injuries included lower extremities in 76 Mean hospital length of stay was 7. Mean injury severity score was 8. Age, injury severity score, and pelvic fracture increased the hospital length of stay. Patients fell while emigrating-immigrating based on residence and motivating factors. A dyad of lower extremity and thoracolumbar spine injuries coincided in Proposed prevention strategies include posting signs on bridges and installing catch-net safety barriers. Harmonization of sound insulation descriptors and classification schemes in Europe: Handbook of noise and vibration control, USA: Wiley and Son; [Ch.
However, by preparing a harmonized European classification scheme with a number of quality classes, member states could select a "harmonized" class fitting the national needs and conditions. The paper will summarize the background, discuss the present situation in Europe and describe the joint efforts to reduce the diversity in Europe, thus supporting and initiating — where needed — improvement of sound insulation of new and existing dwellings in Europe to the benefit Two residues of Escherichia coli elongation factor Tu involved in binding of aminoacyl-tRNA were identified and subjected to mutational analysis.
Lys and Asn were each replaced by either Ala or Glu. Most conspicuously tRNA affinities were reduced for all mutants. The results verify our structural analysis of elongation factor Tu in complex with aminoacyl Their functional roles are discussed in relation to the structure of elongation factor Tu in complex with aminoacyl-tRNA. Towards an understanding of structure-function relationships of elongation factor Tu.
In light of the recently determined structure of elongation factor Tu , and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. An efficient algorithm for nucleolus and prekernel computation in some classes of TU -games. We consider classes of TU -games. We show that we can efficiently compute an allocation in the intersection of the prekernel and the least core of the game if we can efficiently compute the minimum excess for any given allocation.
In the case where the prekernel of the game contains exactly one core. An explicit and universal Darboux transformation for the whole hierarchy is constructed. The soliton solutions for the BPT hierarchy are obtained by applying the Darboux transformation.
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Of these, 51 patients had their conditions diagnosed as PDAC, whereas this diagnosis was ruled out in 52 after 12 months of follow-up. Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of transfer of AMP to target proteins.
Doc from the doc-phd toxin-antitoxin module is a member of the Fic family and inhibits bacterial translation by an unknown mechanism. Here we show that, in contrast to having AMPylating activity, Doc is a new type of kinase that inhibits bacterial translation by phosphorylating the conserved threonine Thr of the translation elongation factor EF- Tu , rendering EF- Tu unable to bind aminoacylated tRNAs.
The results bring insights into the mechanism and role of phosphorylation of EF- Tu in bacterial physiology as well as represent an example of the catalytic plasticity of enzymes and a mechanism for the evolution of new enzymatic activities. DB - Geology ; Mineralogy http: It is not the exchange rate between shekels and drachmas, as was generally assumed, but it is the Babylonian subdivision "counting" of the mina as opposed to the Greek mina.
A Babylonian mina counts 30 staters, a Greek mina Gebremeskel Gebre ; J. He Jiyin ; J. Lin Jimmy ; A. Said Alan ; T. Samar Thaer ; A. Full Text Available This article describes the results obtained from continuous monitoring of fuel flow on the airplanes Tu , operated by the aircompany "Vladivostok Avia". The reasons for the change in the cost of each copy of airplane and changes in fuel characteristics due to pilots manner of flying.
AJ - Letters, Mass-media, Audiovision. On of the main challenges of COST Action TU is to identify, categorize and to the extent possible represent performance indicators of roadway bridges in a manner supporting decision making for their service life integrity management. This calls for an information consistent approach accounting Mutational analysis of Escherichia coli elongation factor Tu in search of a role for the N-terminal region. We have mutated lysine 2 and arginine 7 in elongation factor Tu from Escherichia coli separately either to alanine or glutamic acid.
The aim of the work was to reveal the possible interactions between the conserved N-terminal part of the molecule, which is rich in basic residues and aminoacyl Furthermore, arginine 7 seems to play a role in regulating the binding of GTP. The three-dimensional structure of the ternary complex, EF- Tu: Noncentrosomal microtubule MT nucleation at the Golgi generates MT network asymmetry in motile vertebrate cells.
Using computational simulations, we propose two plausible mechanistic models of GDMT nucleation leading to this phenotype. In the "cooperativity" model, formation of a single GDMT promotes further nucleation at the same site. In the "heterogeneous Golgi" model, MT nucleation is dramatically up-regulated at discrete and sparse locations within the Golgi. While MT clustering in hotspots is equally well described by both models, simulating MT length distributions within the cooperativity model fits the data better. Investigating the molecular mechanism underlying hotspot formation, we have found that hotspots are significantly smaller than a Golgi subdomain positive for scaffolding protein AKAP, which is thought to recruit GDMT nucleation factors.
This article is distributed by The American Society for Cell Biology under license from the author s. Two months after publication it is available to the public under an Attribution—Noncommercial—Share Alike 3. The novel, published in , was among the best seller until s. In addition to its popularity among the youth and the children, this work of literature still has effects on contemporary intellectuals and politicians. The author focuses on the importance of the Turkish Union while narrating the war between Genghis Khan and China.
Could intervention by clinics such as Primeros Pasos affect the prevalence of childhood malnutrition in the developing world? Guatemala is a country severely affected by malnutrition, particularly in its child population. The present study aimed to assess the impact of recognised aetiological factors on the prevalence of malnutrition and to establish the efficacy of a Guatemalan malnutrition clinic by examining the changes in the prevalence of malnutrition in the ten surrounding communities served by the clinic.
Records of children n aged years seen by the clinic between and were examined. Anthropometric data for each child were taken from these records and used as an indicator for their nutritional status to estimate the change in rates of malnutrition during this period. A consistent reduction was also seen in rates of moderate and severe malnutrition, but not in rates of mild malnutrition.
The results demonstrate that clinics such as Primeros Pasos can have a beneficial effect on schoolchildren, particularly those who are severely malnourished. A need has been highlighted for more research in this area, particularly how clinics such as Primeros Pasos can be best used in the fight against malnutrition.
This paper focuses on the position of stepping motors in current industrial applications, recent advances andgeneral trends in design and manufacturing such motors. Their most outstanding features and different typesare also shown. Stepping motors, motion control. This Environmental Assessment documents the analysis of alternative corridors for development and operation of a proposed kilovolt transmission line using private lands and transporting power to the US-Mexico international border.
The alternative corridors, specific locations within those corridors, and structure types are identified and analyzed in the environmental studies. Proyecto del paso superior sobre la carretera M y accesos. Enlace con Villanueva del Pardillo en el P. Function and structure in phage Qbeta RNA replicase. Association of EF- Tu -Ts with the other enzyme subunits. This conformational change is not influenced by ionic strength. The addition of Qbeta RNA to the enzyme, does not result in the release The enzyme is capable of initiation of RNA synthesis with synthetic templates only when in the low ionic strength conformation.
Elongation of initiated polynucleotide chains is not affectedby ionic strength. Addition of Qbeta RNA to the enzyme also Duplication of Drosophila melanogaster mitochondrial EF- Tu: Mitochondria of the enoplean nematode Trichinella possess three structural types of tRNAs: We report here that two EF- Tu species also participate in Drosophila melanogaster mitochondria. These results suggest that the Drosophila mt translation system could be another intermediate state between the canonical and nematode mitochondria-type translation systems.
Translation elongation factor EF- Tu modulates filament formation of actin-like MreB protein in vitro. EF- Tu has been shown to interact with actin-like protein MreB and to affect its localization in Escherichia coli and in Bacillus subtilis cells. We have purified YFP-MreB in an active form, which forms filaments on glass slides in vitro and was active in dynamic light-scattering assays, polymerizing in milliseconds after addition of magnesium.
Purified EF- Tu enhanced the amount of MreB filaments, as seen by sedimentation assays, the speed of filament formation and the length of MreB filaments in vitro. EF- Tu had the strongest impact on MreB filaments in a 1: This was supported by cross-linking assays where 1: When expressed in E.
Fluorescence recovery after photobleaching analysis showed that MreB filaments have a higher turnover in B. The data show that MreB can direct the localization of EF- Tu in vivo, which in turn positively affects the formation and dynamics of MreB filaments. Thus, EF- Tu is a modulator of the activity of a bacterial actin-like protein.
Published by Elsevier Ltd. Comparative measurement of sup 99m Tc-thyroid-uptake Tc TU using two different methods of data analysis. A new program for automatic measurement of Tc TU was developed and compared with a standard interactive method of data analysis. The new program fullfils the requirements for correct calculation of Tc TU. The El Paso Children's Health Study examined ambient exposures to motor vehicle emissions and their effect on the prevalence of allergy and asthma among children living in a major United States-Mexico border city. In the spring of , the parents of all fourth and fifth grad Diez pasos para el aprendizaje complejo: Aseguramiento de la Calidad en la Educacion y en el Trabajo.
Geophysical survey, Paso Robles geothermal area, California, part of the resource assessment of low- and moderate-temperature geothermal resource areas in California. Some general background information concerning the geology and geothermal occurrences in the Southern Coast Ranges is included, as well as the more detailed information dealing with the Paso Robles area proper.
Results for two geophysical methods that have been used in the area: To examine the association between nutrition knowledge and eating behavior in a predominantly Mexican American population on the Texas-Mexico border. Cross-sectional using data from the baseline survey of the Que Sabrosa Vida community nutrition initiative. El Paso and surrounding counties in Texas.
InTeGrate Interdisciplinary Teaching about Earth for a Sustainable Future has developed teaching modules that focus on Earth sustainability and Earth-centered societal issues.
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Our eventual goal is to insure students taking introductory classes at any institution will be exposed to comparable content and be similarly prepared for advanced courses. Since many students do not speak English at home, they were challenged by vocabulary presented in some modules. Modules containing glossaries and extensive background material such as concept maps and annotated figures proved very helpful to these students. The use of pre-activity quizzes insured that the students had mastered basic concepts needed for in-class activities.
Student reflections indicated students were able to make connections to societal issues and retain these ideas through the end of the courses. Bacterial elongation factors EF- Tu , their mutants, chimeric forms, and domains: Substitution route has been traditionally proposed for the synthesis of new Tc complexes. In this substitution reaction, TcO 2 is a side product that should be minimized.
The success of these synthetic procedures is based on controlling the reaction conditions in order that pure substitution could be faster enough compared to decomposition. As a general conclusion, substitution constants are strongly dependent on the pH. No strong differences are found with different incoming ligands.
Arabidopsis EF- Tu receptor enhances bacterial disease resistance in transgenic wheat. Previous work revealed that transgenic expression of AtEFR in Solanaceae confers elf18 responsiveness and broad-spectrum bacterial disease resistance. We generated transgenic wheat Triticum aestivum plants expressing AtEFR driven by the constitutive rice actin promoter and tested their response to elf We show that transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition.
When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. These results demonstrate that AtEFR can be transferred successfully from dicot to monocot species, further revealing that immune signalling pathways are conserved across these distant phyla. As novel PRRs are identified, their transfer between plant families represents a useful strategy for enhancing resistance to pathogens in crops.
Paso a Paso Step by Step. This podcast encourages people to increase physical activity with original music with a Latino beat. The results corroborate an earlier inference that all four sites can simultaneously tether ternary complexes near the A site, creating a high local concentration that may greatly enhance the rate of testing of aa-tRNAs. The results refute a recent study A. Kapanidis, Nucleic Acids Res Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR.
Here, the transgenic rice plants Oryza sativa L. We demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa-tion in the AtEFR transgenic rice cel s and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatical y in transgenic rice plant and cel lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig-gered strong resistance to X.
These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Col ectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF- Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.
RT I nr. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination EF-Ts directly facilitates the formation and disassociation of ternary complex. This system demonstrates a novel function of EF-Ts.
Here, we describe bulk steady state and pre-steady state fluorescence methods that enabled us to quantitatively explore the kinetic features of Escherichia coli ternary complex formation and decay. The data obtained suggest that both processes are controlled by a nucleotide-dependent, rate-determining conformational change in EF- Tu. Unexpectedly, we found that this conformational change is accelerated by elongation factor Ts EF-Ts , the guanosine nucleotide exchange factor for EF- Tu. These results suggest that EF-Ts serves an unanticipated role in the cell of actively regulating the abundance and stability of ternary complex in a manner that contributes to rapid and faithful protein synthesis.
The article is structured in three parts, as follows: Mutation of the conserved Gly94 and Gly in elongation factor Tu from Escherichia coli. Elucidation of their structural and functional roles.
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All guanine-nucleotide-binding proteins cycle between an inactive, GDP-bound and an active, GTP-bound conformation whereby they function as molecular switches. Elongation factor Tu from Escherichia coli is used as a model for defining residues important in the switch mechanism. Both mutations affect the affinities for guanine nucleotides considerably, resulting in a decrease in the characteristic preference The aminoacyl-tRNA affinity is much reduced for [G94A]EF- Tu , as reflected in an increase of the dissociation rate constant for the ternary complex by a factor of Surprisingly, however, both mutants It is localized at the microtubule organizing centers and mitotic spindle fibres.
This template thus determines the resulting microtubule lattice. Our free energy decomposition data from converting a compendium of amino acid residues identified an array of hotspot amino acids. A subset of such mutants can be expressed in vivo in living yeast. Paso del Foso de las Murallas — Francia. Full Text Available The Western Bypass Road at Strassbourg is a wide motor road, which runs around the city and linics the northern and sourthem motor roads. This ensures the continuity of the motor road network in the vincinity of Strassbourg's urban zone, and also facilitates communications with the city centre.
The motor road crosses the moat next to the city walls near the railway crossing, this being achieved by means of prefabricated reinforced concrete boxes. The objective was to identify and save plant resources of cultural interest, in addition to discuss the community and educational processes that promote participatory research citizen science with local actors. Five workshops were held on the different themes of ethnobotany, such as environmental interpretation, local productive practices, traditional food, veterinary medicine and forage plants. In the context of the local cultural context, we registered a total of 88 uses in 49 wild species and 3 cultivated species; 7 uses are associated with food, 19 with veterinary medicine, 32 with forage, and 30 with other uses.
University students participated in the organization, planning, and preparation of the resources used as well as fieldwork registration and systematization of workshops. The study discusses how the construction of a dialogical experience promotes greater reciprocity in the process of collection of ethnobotanical data and knowledge, resulting in an educational and pedagogical process. Organic geochemical investigation and coal-bed methane characteristics of the Guasare coals Paso Diablo mine, western Venezuela.
The aim of this work was to carry out a geochemical study of channel samples collected from six coal beds in the Marcelina Formation Zulia State, western Venezuela and to determine experimentally the gas content of the coals from the Paso Diablo mine. Organic geochemical analyses by gas chromatography-mass spectrometry and isotopic analyses on-line in coalbed gas samples were performed.
The results suggest that the Guasare coals were deposited in a continental environment under highly dysoxic and low salinity conditions. The non-detection of 18?? H -oleanane does not preclude that the organic facies that gave rise to the coals were dominated by angiosperms. In addition, the presence of the sesquiterpenoid cadalene may indicate the subordinate contribution of gymnosperms conifers in the Paleocene Guasare mire. The average coalbed gas content obtained was 0. The El Paso Formations consists of four members, in ascending order: Most of these mounds are small but one large one is 5.
The mound rock is chiefly bioclastic wackestone with minor packstone and boundstone. The varied fauna contains echinoderms, sponges and spicules, gastropods, trilobites, digitate algae, Nuia, Girvanella, Pulchrilamina, Calathium, and minor brachiopods and cephalopods. Intraclastic, bioclastic grainstone fills channels cut in the mounds. These mounds also are typically small but one in the Red Hills is The limited fauna contains small fragments of echinoderms, gastropods, trilobites, spicules, and Nuia.
Soil-borne polycyclic aromatic hydrocarbons in El Paso , Texas: The method provided good sensitivity and faster processing time for the analysis. Although the majority of PAH concentrations did not exceed the soil screening levels regulated by the United States Environmental Protection Agency, the existence of PAHs in this ecosystem is ubiquitous. The results ruled out the possibility of petroleum refining as the significant source of local soil-borne PAH contamination, but they suggested that the PAHs found in El Paso soil were closely linked to human activities and possible other industrial processes.
Full Text Available Purpose. Crohn's disease CD and ulcerative colitis UC, the two major forms of inflammatory bowel disease IBD, have histopathologically and immunologically different characteristics. We previously reported that a traditional Japanese medicine, daikenchuto TU , ameliorated a trinitrobenzenesulfonic acid- TNBS- induced type-1 model colitis exhibiting histopathological features of CD through adrenomedullin ADM enhancement.
Our current aims were to examine whether TU ameliorates a type-2 model colitis that histologically resembles UC and identify the active ingredients. TU was administered orally to mice with oxazolone- OXN- induced type-2 model colitis. The morbidity was evaluated by body weight loss and the macroscopic score of colonic lesions.
TU prevented weight loss and colon ulceration.
ADM production by intestinal epithelial cells was increased by TU addition. The possibility is studied of real time testing for the content of mechanical impurities Cm. Based on the obtained data, a four point scale of gray standards is developed for determining the mechanical impurity content, which is four rounded, gray stamps of different intensity, which corresponds to a mechanical impurity content of 0.
A white indicator filtering element is built into the POZ- TU for determining the mechanical impurity content, and 50 cubic centimeters of the jet fuel are pumped through it over the course of several seconds. The mechanical impurities are placed on the indicator element, forming an imprint, the intensity of the color of which corresponds to the content of mechanical impurities in the jet fuel. The indicator element is extracted from the instrument and the prints are compared with the scale of gray standards, from which the content of the mechanical impurities is determined.
The "Da Ming Hunyi Tu ": In official statements and popular media coverage alike, the map was described as evidence of a distinctly Chinese approach to global relations, based on benevolence and mutual respect. This article examines the ways in which the map has been divorced from its original context to suit modern needs, exemplifying the deployment of cartography to deflect anxieties about the nature of Chinese economic influence in South Africa.
Developing a tissue bank database has become more than just logically arranging data in tables combined with a search engine. Current demand for high quality samples and data, and the ever-changing legal and ethical regulations mean that the application must reflect Tu BaFrost rules and protocols for the collection, exchange and use of tissue. To ensure continuation and extension of the Tu BaFrost European tissue bank, the custodianship of the samples, and hence the decision over whether to issue samples to requestors, remains with the local collecting centre.
The database application described in this article has been developed to facilitate this open structure virtual tissue bank model serving a large group. It encompasses many key tasks, without the requirement for personnel, hence minimising operational costs. The Internet-accessible database application enables search, selection and request submission for requestors, whereas collectors can upload and edit their collection. Communication between requestor and involved collectors is started with automatically generated e-mails.
Type VI secretion T6S influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis.
We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu EF- Tu. These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria. Full Text Available Background: Ethnobotany studies the knowledge produced by the interaction between society and the use of plants that have been transmitted between generations and are still preserved in the different sectors of the rural community of the El Hatillo, Jurisdiction of La Loma de calentura, Town of El Paso , Cesar.
Currently, very few ethnobotanical studies have been done in Colombia: Spread of knowledge about the use of plants by characteristics of the informants, use of plants by farmer groups in the tropical belt of Paramillo National Natural Park, and utility and value in use of ethnobotany, a study in the state of Putumayo, among others. Therefore, there is a need for further studies to be done throughout the Colombian territory, as many of these communities are currently being affected by mining and military activities among others.
This research was conducted with the aim of rescuing the ethnobotanical knowledge. For collection, classification and preservation of popular ethnobotanical knowledge, tools like the plant spreadsheet model, community interviews, focus groups, pictures, videos, audio and taxonomic samples were used. The most common use was medicinal, the most used part is the leaf, and the way of preparation is cooking. Currently, this community must to be be relocated because it is affected by the direct environmental impact due to coal dust release as well as vibrations due to mining blast, which affects surface waters, groundwater and soil composition.
Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. A tRNA containing a long extra arm, namely E. The nucleotide involved in the crosslinking was identified to be a guanosine in the variable region at position 47F or 47G Mitochondrial translation factors of Trypanosoma brucei: The surface-associated elongation factor Tu is concealed for antibody binding on viable pneumococci and meningococci.
The assemblage from the lower level is considerably less diverse than that from the upper level. It contains Progonomys together with. A guidelines handbook for GPR surveys in tunnels: A significant open issue concerning the reliability of geophysical methods and in particular of ground penetrating radar GPR , both in research and professional context, is a general lack of international standards. This is a major problem to be faced, in order to gain scientific strictness for the GPR practices, and to easily extend to the international community the results achieved within the area of single virtuous countries.
Producing international guidelines can represent an important step forward, in this sense. In the memorandum of understanding of the COST Action TU is clearly stated that one of the main purposes of the Action is the "development of innovative protocols and guidelines which will be published in a handbook and constitute a basis for European Standards, for an effective GPR application in CE tasks; safety, economic and financial criteria will be integrated within the protocols".
Of course this is not a simple task to be accomplished. Firstly, survey procedures are highly dependent on the objective of the survey itself. On the basis of the objective of each geophysical test, the GPR system, the antenna configuration, and even the processing procedures may change. Besides, these procedures are also influenced by the environmental conditions in which the tests are performed.
This affects several aspects spanning from hardware to software, but including, for instance, also safety issues. Due to these reasons, one of the main goal of the COST Action TU is the development of several guidelines related to the main applications of GPR in the field of civil engineering. In this work, the structure of a guidelines handbook for GPR activities in tunnels is outlined. In the first sections, the principal references in the field are provided, and the most common GPR equipment and complementary technologies are described.
Subsequently, the survey methodologies are explained. Particular attention is paid to the preliminary activities to be carried. Use of neutron activation analysis to determine arsenic and antimony concentrations in creosote bushes collected near a lead smelter in El Paso , Texas. These concentrations are at least one order of magnitude higher than naturally occurring levels.
The objective of this work is to determine the amount of metal accumulation within creosote bush, as it is found naturally growing in metal contaminated soils through analysis of soil and plant tissue samples. Background Phytoplasmas are bacterial phytopathogens responsible for significant losses in agricultural production worldwide. Several molecular markers are available for identification of groups or strains of phytoplasmas. However, they often cannot be used for identification of phytoplasmas from DNA barcoding recently emerged as a convenient tool for species identification.
Here, the development of a universal DNA barcode based on the elongation factor Tu tuf gene for phytoplasma identification is reported. Comparison of NJ trees constructed from the tuf barcode and a 1. Full Text Available The fight for viewers has led to a spectacular range of television content on offer where boundaries of genres have disappeared giving rise to new formats of hybrid television programmes.
This has given a new lease of life to interviews as a genre of infotainment. Sound insulation descriptors, regulatory requirements and classification schemes in Europe represent a high degree of diversity. One implication is very little exchange of experience of housing design and construction details for different levels of sound insulation; another is trade barriers Unfortunately, there is evidence for a development in the "wrong" direction.
For example, sound classification schemes for dwellings exist in nine countries. There is no sign on increasing harmonization, rather the contrary, as more countries are preparing proposals with new Housing for the poor: Implementation and impact of Tu Casa program in Tijuana. The exam aims to analyses the design changes of the program that forced its transformation from Vivah to Tu Casa, and the operational difficulties for a proper implementation in an urban context, as well as its limited impact for solving popular housing needs.
It also seeks to weigh the importance of government subsidies to acquire or improve housing, in their progressive modality, as an alternative for social segments excluded from any other public or private financing option. Full Text Available http: Full Text Available Equine degenerative suspensory ligament desmitis DSLD in Peruvian Paso horses typically presents at years and is characterized by lameness, focal disorganization of collagen fibrils, and chondroid deposition in the body of the ligament.
Heritage tourism businessmen network Ciudad Juarez-El Paso. SHM research and engineering has been focused on the extraction of loading, degradation This challenge can be met with Value of SHM Information analyses facilitating that the SHM contribution to substantial benefits for life safety, economy and beyond can be may be quantified, demonstrated and utilized.
An integrable coupling family of Merola-Ragnisco- Tu lattice systems, its Hamiltonian structure and related nonisospectral integrable lattice family. An integrable coupling family of Merola-Ragnisco- Tu lattice systems is derived from a four-by-four matrix spectral problem. The Hamiltonian structure of the resulting integrable coupling family is established by the discrete variational identity. Each lattice system in the resulting integrable coupling family is proved to be integrable discrete Hamiltonian system in Liouville sense.
Ultimately, a nonisospectral integrable lattice family associated with the resulting integrable lattice family is constructed through discrete zero curvature representation. Geer, van der S. Thermostability of Multidomain Proteins: Behaviour of hormone parameters and equivalent iodide clearance Tc Tu on suppressive T4 treatment of enthyroid struma.
After 8 to 12 weeks of therapy, the Tc TU as an estimate of the iodide clearance was suppressed to 0. T 4 and NTR values were near the upper limit of the normal at a normal T 3 level. Therapy was generally less successful in patients over 18 years of age. Geological Survey in cooperation with the U. The landfill contains household refuse, Post solid wastes, bulky items, grass and tree trimmings from family housing, refuse from litter cans, construction debris, classified waste dry , dead animals, asbestos, and empty oil cans. The MSWLF, located about 1, feet east of the nearest occupied structure, is estimated to receive an average of approximately 56 tons of municipal solid waste per day and, at a fill rate of acres per year, is expected to reach its capacity by the year The climate at the MSWLF and vicinity is arid continental, characterized by an abundance of sunny days, high summer temperatures, relatively cool winters typical of arid areas, scanty rainfall, and very low humidity throughout the year.
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A pseudonym of Elisabetta Dami , aka Thea Stilton. Elisabetta Dami is an Italian author that currently resides in Italy. She is the author of the Geronimo Stilton series, a bestselling childrens book. She enjoys traveling the world seeking ideas for her series. She writes under a pseudonym of Geronimo Stilton. She is also a co-owner of Italian based publishing company Edizioni Piemme. Her books have been translated into English by Scholastic Inc. Other books in the series.