ELISA Enzyme linked immunosorbent assay is a biological analysis technique based on the immunoreaction of antigen and antibody and the efficient catalytic reaction of enzyme. It can be used for qualitative and quantitative analysis of target protein in the sample like serum and plasma. A complete ELISA kits which contains all the reagents that you can use in your experiments, its corresponding experimental operation can refer to the instruction manual directly.
For the sample to be tested, you can get the preliminary judgement by pre-experiment.
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For example, whether the sample needs to be diluted or not. Please refer to the instruction manual for the preparation of the sample.
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If it is not mentioned, please contact us Just click "chat now" in the lower right corner. All the reagents used should be prepared according to the requirements of the specification. Pre-experiment generally set up 2 strips 16 wells fig ,. The sample to be tested was brain tissue K1, M1 random number. Different dilution times were selected for pre-experiment. The corresponding OD values are as follows:. Using Curve Expert 1. For details of the drawing process, please refer to: You can obtain the concentration of the sample to be tested by putting the OD value of the sample into the formula.
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the Pre-experiment ofELISA-CUSABIO
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