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The downside, however, is that quantum dots are usually made of quite toxic elements, but this concern may be addressed by use of fluorescent dopants. Tracking movement can help determine how well drugs are being distributed or how substances are metabolized. It is difficult to track a small group of cells throughout the body, so scientists used to dye the cells. These dyes needed to be excited by light of a certain wavelength in order for them to light up. While different color dyes absorb different frequencies of light, there was a need for as many light sources as cells.

A way around this problem is with luminescent tags. These tags are quantum dots attached to proteins that penetrate cell membranes. As a result, sizes are selected so that the frequency of light used to make a group of quantum dots fluoresce is an even multiple of the frequency required to make another group incandesce. Then both groups can be lit with a single light source. They have also found a way to insert nanoparticles [38] into the affected parts of the body so that those parts of the body will glow showing the tumor growth or shrinkage or also organ trouble.

Nanotechnology-on-a-chip is one more dimension of lab-on-a-chip technology. Magnetic nanoparticles, bound to a suitable antibody, are used to label specific molecules, structures or microorganisms. In particular silica nanoparticles are inert from the photophysical point of view and might accumulate a large number of dye s within the nanoparticle shell. Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots into polymeric microbeads. Nanopore technology for analysis of nucleic acids converts strings of nucleotides directly into electronic signatures.

Sensor test chips containing thousands of nanowires, able to detect proteins and other biomarkers left behind by cancer cells, could enable the detection and diagnosis of cancer in the early stages from a few drops of a patient's blood. The smaller the incisions the faster the healing time which is better for the patients. It is also helping to find a way to make an arthroscope smaller than a strand of hair.

Research on nanoelectronics -based cancer diagnostics could lead to tests that can be done in pharmacies. The results promise to be highly accurate and the product promises to be inexpensive. They could take a very small amount of blood and detect cancer anywhere in the body in about five minutes, with a sensitivity that is a thousand times better a conventional laboratory test. These devices that are built with nanowires to detect cancer proteins; each nanowire detector is primed to be sensitive to a different cancer marker. They have found ways that they will be able to target a specific part of the body that is being affected by cancer.

Magnetic micro particles are proven research instruments for the separation of cells and proteins from complex media. The technology is available under the name Magnetic-activated cell sorting or Dynabeads among others. More recently it was shown in animal models that magnetic nanoparticles can be used for the removal of various noxious compounds including toxins , pathogens , and proteins from whole blood in an extracorporeal circuit similar to dialysis.

Additionally larger compounds which are commonly not dialyzable can be removed. The purification process is based on functionalized iron oxide or carbon coated metal nanoparticles with ferromagnetic or superparamagnetic properties. These binding agents are able to interact with target species forming an agglomerate. Applying an external magnetic field gradient allows exerting a force on the nanoparticles. Hence the particles can be separated from the bulk fluid, thereby cleaning it from the contaminants.

These advantages are high loading and accessible for binding agents, high selectivity towards the target compound, fast diffusion, small hydrodynamic resistance, and low dosage. This approach offers new therapeutic possibilities for the treatment of systemic infections such as sepsis by directly removing the pathogen. It can also be used to selectively remove cytokines or endotoxins [48] or for the dialysis of compounds which are not accessible by traditional dialysis methods. However the technology is still in a preclinical phase and first clinical trials are not expected before Nanotechnology may be used as part of tissue engineering to help reproduce or repair or reshape damaged tissue using suitable nanomaterial-based scaffolds and growth factors.

Tissue engineering if successful may replace conventional treatments like organ transplants or artificial implants. Nanoparticles such as graphene, carbon nanotubes, molybdenum disulfide and tungsten disulfide are being used as reinforcing agents to fabricate mechanically strong biodegradable polymeric nanocomposites for bone tissue engineering applications.

For example, a flesh welder was demonstrated to fuse two pieces of chicken meat into a single piece using a suspension of gold-coated nanoshells activated by an infrared laser. This could be used to weld arteries during surgery. Neuro-electronic interfacing is a visionary goal dealing with the construction of nanodevices that will permit computers to be joined and linked to the nervous system. This idea requires the building of a molecular structure that will permit control and detection of nerve impulses by an external computer.

A refuelable strategy implies energy is refilled continuously or periodically with external sonic, chemical, tethered, magnetic, or biological electrical sources, while a nonrefuelable strategy implies that all power is drawn from internal energy storage which would stop when all energy is drained. A nanoscale enzymatic biofuel cell for self-powered nanodevices have been developed that uses glucose from biofluids including human blood and watermelons.

The wiring of the structure is extremely difficult because they must be positioned precisely in the nervous system. The structures that will provide the interface must also be compatible with the body's immune system. Molecular nanotechnology is a speculative subfield of nanotechnology regarding the possibility of engineering molecular assemblers , machines which could re-order matter at a molecular or atomic scale. Nanomedicine would make use of these nanorobots , introduced into the body, to repair or detect damages and infections.

Molecular nanotechnology is highly theoretical, seeking to anticipate what inventions nanotechnology might yield and to propose an agenda for future inquiry. In addition, they can encapsulate solutes which have different properties [ 27 ]. The dendrimer term, named from their structural shape, was firstly proposed by Donald Tomalia and his co-workers in the early s.

Dendrimers are monodisperse symmetric macromolecules with highly branched structures around an inner core [ 12 ]. Their structures are comprised of three components: The terminal groups of dendrimers mostly control the dendrimer interactions with the molecular environment. The interior of a dendrimer can show hydrophilic characteristics while the exterior surface of a dendrimer is hydrophobic or vice versa by modifying their termini. Their properties such as nanometre size range, ease of preparation and functionalisation, also their multiple copies of surface groups displaying ability, make them an attractive system for drug delivery [ 12 ].

Because of their non-polar cavities, they can encapsulate hydrophobic drug molecules. In addition, they have many positively and negatively charged functional groups on their surface which offers the opportunity to easily attach to oppositely charged drug molecules [ 10 ]. There were two methods using dendrimers for drug delivery: In the encapsulation method, drug molecules were entrapped into dendrimers. In the conjugation method, drug molecules would be covalently attached onto the surface of the dendrimer [ 10 ].

Carbon nanotubes CNTs are attractive systems because of their excellent mechanical, electrical and surface properties. Surface properties, size and shapes of CNTs are several factors which affect interactions with cells. They need to be functionalised due their insolubility in most types of solvents and their cytotoxic properties. Their needle-like shape offers the opportunity to facilitate transmembrane penetration [ 28 ]. The nanotube diameter, degree of chirality and being single walled or multiwalled are some of the factors that affect the properties of the nanotubes.

They provide several approaches for drug delivery. Nanocrystals are molecule aggregates which comprise the crystalline drug [ 30 , 31 ]. They are especially used for poorly soluble drug molecules. They can be formulated as suspensions by dispersing drug molecules in a liquid medium which are called nanosuspensions and also dry dosage forms such as tablets and capsules [ 31 , 32 ]. They have several advantages for drug delivery. They can provide enhanced bioavailability by improving solubility and bioadhesion to the intestinal wall.

Hydrogels can be defined as three-dimensional hydrophilic structure networks which are formed chemically or physically. They have some properties which must be optimised such as safety, biodegradability, drug loading capacity and drug-release kinetics [ 33 ]. Drug molecules can be loaded by their porous structure which can be controlled by the density of cross-links.

The drug-release rate is determined by the diffusion coefficient of the drug molecule [ 34 ]. Hydrogels can be programmed to alter their structure by environmental changes such as pH and temperature which are called smart hydrogels. Hence, they can be used for developing stimuli-responsive drug delivery systems. They also give us a chance to develop biomimetic systems [ 35 ]. Nanotechnology is a very promising and open-ended area for drug delivery.

It has great potential to innovate drug delivery systems and offers new opportunities to design variable and game-changing systems which can be adapted for drugs. The area of nanotechnology is still evolving and continuing to grow. Also, it provides a wide variety of nanomaterials which can be used to produce effective drug delivery systems with desirable properties. There are numerous types of drug delivery systems associated with nanotechnology which gives us a chance to select the system which has desirable properties for our purpose from among these various systems.

However, there are some drawbacks in using nanosystems.

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Stability of nanocarriers is difficult because of their large surface area. Additionally, the charactheristics of nanocarrier systems must be fully understood to achieve optimum in vivo efficiacy. Long-term toxicity and stability must be studied before using them in human health care. Behaviours of nanoparticles should be investigated in the human body. They may trigger blood coagulation pathways and may cross the physiological barriers such as blood-brain barrier unintentionally.

Also, the toxicity parameters of bulk material can be changed by using them in a nanoformulation. Therefore, toxicity parameters must be examined before they generate formulation for all ingredients which are used in nanoscale. In spite of their drawbacks, the advantages of nanosystems are incontestable. Nanotechnology is extremely important for future medicine.

Nanotechnology-mediated drug delivery systems offer many solutions for potential therapeutic agents which cannot be used properly due to their characteristics such as low solubility and low bioavailability. Therefore, there is much to be done with nanosystems use them more efficiently and safely for human health care. All authors contributed to the conception, design, and preparation of the manuscript, as well as read and approved the final manuscript. Nanosystems for drug delivery. Abstract Introduction Over the last couple of decades, the area of drug delivery has become important for its possible gaining in the pharmaceutical industry.

Conclusion Novel drug delivery systems can enhance important characteristics of drugs such as bioavailability and drug solubility.

Nanomedicine: Nanotechnology, Biology and Medicine - Journal - Elsevier

Introduction Biodegradable nanoparticles NPs have played an important role for developing new drug delivery systems in recent years. Market forecasts for nanotechnology between and in billions of US dollars. Advantages of nanocarriers Polymeric NPs which are made from natural and synthetic polymers were generated to achieve controlled drug release and targeting. Effecting parameters of nanoparticular drug delivery Particle size The most important characteristics of NPs are particle size and size distribution due to their direct impact on in vivo distribution, biological fate, toxicity and targeting ability as well as drug loading, drug release and stability of NPs.

Surface properties of nanoparticles In vivo fate of NPs can be determined by their hydrophobicity. Drug loading and drug release High drug loading capacity is the necessity for a successful nanodelivery system. Nanosystems for drug delivery Inorganic NPs Inorganic NPs are metal oxide particles or the particles which possess at least one metallic composition at nanoscale.

Nanospheres Polymeric nanospheres are matrix-type solid colloidal particles. Nanocapsules Nanocapsules are nano-vesicular systems in which drugs are enclosed to a cavity, surrounded by unique polymer membrane or coating. Polymeric micelles Polymeric micelles have unique amphiphilic properties with a core-shell structure.

Liposomes Liposomes are spherical shaped artificial vesicles which are produced by natural non-toxic phospholipids and cholesterol. Dendrimers The dendrimer term, named from their structural shape, was firstly proposed by Donald Tomalia and his co-workers in the early s. Carbon nanotubes Carbon nanotubes CNTs are attractive systems because of their excellent mechanical, electrical and surface properties. Nanocrystals Nanocrystals are molecule aggregates which comprise the crystalline drug [ 30 , 31 ].

It is working in the direction of making possible the detection of a single ill cell and curing or eliminating it. In vivo imaging devices and tools using nanotechnology are constantly being developed [8]. Following are the techniques used which help in the detection of tumors in the body: Nanowires- There is a microfluidic channel across which the nano sized sensing wires or nanowires are arranged.

When particles pass through this channel, the sensors on the nanowires pick up the specific molecular signatures of these particles. This information is then transmitted instantly through a system of electrodes to the researcher. They detect the presence of transformed genes associated with cancer. This further helps in identifying the precise site of those modifications [9]. Cantilevers- These are beams coated with molecules capable of binding specific substrates. Such micronsized devices, can detect single molecules of DNA or protein.

As a cancer cell secretes proteinaceous molecular products, the antibodies bound on the surface of the cantilever fingers selectively combine with these secreted proteins. These antibodies have been fabricated such that they can pick up one or more, distinctive molecular expressions from a malignant cell. The binding event results in change in the conductance of the cantilevers, thus helping in rapid and sensitive detection of cancer-related molecules [10].

Nanoparticles- Nanoparticles are directed to cancer cells to aid in the imaging of a cancerous tumor at the molecular level. Large numbers of radiolabelled nanoparticles are safely injected into the body, which bind favorably to the cancer cell, thus making it visible. Thus nanoparticles give us the ability to see cells and molecules that cannot be otherwise detected through conventional imaging.

This capability to gather what happens inside the cell - to observe therapeutic intervention and to see when a cancer cell is lethally injured or is actually stimulated - is crucial to the effective diagnosis and treatment of the disease [5]. Nanoparticles may be of biological or chemical origin. Biological materials include phospholipids, lipids, chitosan, dextran and lactic acid. Chemical nanoparticles may be made of carbon, silica, polymers or metals like cadmium or iron [3].

Carbon nanotubes- These are long, needle-like, C fullerene-based tubes that act as bio-persistent fibers. They help to identify the exact location of the cancer-related DNA changes, thereby aiding in diagnosis of the disease.

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The mutated areas linked with cancer are tagged with bulky molecules. This is followed by tracing the physical shape of the DNA using the nanotube tip, which is then digitally translated into a topographical map. With the help of the bulky molecules, the precise location of the mutations can be pinpointed on the map [11].

Quantum dots- Quantum dots are nanoscale, coated crystals that are made of a semiconductor material. Their size ranges from 2. These crystals glow when activated by ultraviolet light and the emitted color depends on their size. When they are connected to a molecule that can bind to the substance of interest, they light up when such binding does occur. Therefore, they can light up the sequence under investigation. Because of the various colors and intensities of light that can be emitted by quantum dots, they can be combined to create probes that simultaneously detect several substances, for example, they have been used for the concurrent imaging of multiple proteins [3, 11, 12].

This is being achieved by developing nanoscale particles or molecules to improve the pharmacokinetic and pharmacodynamic properties of a drug. Nanotechnology can be used to overcome the poor bioavailability of drugs. Bioavailability is one of the key pharmacokinetic properties of drugs. When the drug is administered in a dose, the part of the drug that reaches the systemic circulation unchanged, is called its bioavailability.

It is approximated that about 65 billion dollars are being wasted per year because of the poor bioavailability of drugs. That is why novel ways of drug administration are required. Using nano-drug delivery systems significantly improves drug delivery. Being very small in size, nanoparticles are taken up by the cells, unlike other bigger elements, which are discarded that could force a patient to take high doses [13].

On the other hand with nanoparticles, prolonged, targeted action of the drug can be achieved, possibly reducing the drug dose. Although the goal of nanotechnology is to increase bioavailability, in some cases the bioavailability and the drug activity may decrease, like in the case of insulin-chitosan nanoparticles [3]. Problems such as issues related to solubility of drugs can also be solved using nanotechnology as there is reduction in particle size of the poorly water-soluble drugs. In addition, there is benefit of increased active agent surface area.

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Moreover, nano-drugs have the advantage of faster dissolution, which leads to greater bioavailability, smaller drug doses, diminished toxicity and decreased dosing variability [14]. Efforts are constantly being made to develop drug delivery mechanisms, including the ability to transport drugs across cell membranes into the cytoplasm. This is significant because many diseases can be impeded by drugs that can cross through the membrane [13]. Potential nanodrugs will work by very specific and well-understood mechanisms. The drug release may be activated by degradation of the particle, or heat and light may be used for inducing the therapeutic effect.

One of the major impacts of nanoscience will be in development of completely new forms of drugs with more useful behavior, specific targeting, lesser side effects, greater safety and quicker development of new medicines. For this, long-lived nanoformulations will be needed. PEGylation, which is the surface treatment with polyethylene glycol PEG , of nanoparticles results in prolonged presence of the nanoparticles in circulation [3, 15].

Nanoshells- A nanoshell is a type of spherical nanoparticle. It consists of a dielectric center which is covered by a thin metallic shell usually gold [16]. The nanoshells can be injected without harm.


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They collect favourably in cancer wound sites because of their size. This physical selectivity arises due to the enhanced permeation retention EPR phenomenon. Tumour cells express antigens themselves or the antigens are expressed by the tumour microenvironment. Nanoshells can be modified to carry molecular conjugates to these antigens.

This further increases the specificity of nanoshells to preferentially link to the tumour and not to adjoining healthy cells. Then, externally energy can be supplied to these cells.


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  8. The specific properties of the nanoshells allow for this directed energy to be absorbed. This creates an intense heat that kills only the tumor cells. The therapeutic effect is the same irrespective of the form of external energy- whether it be mechanical, radio frequency or optical. The use of nanoshells thus, significantly reduces side effects and increases the efficacy of the treatment [17]. The flexible branches of a dendrimer provide a tailored sanctuary containing voids that provide protection from the outside environment wherein drug molecules can be physically trapped.

    This trapped drug is then released at the tumor site [18]. Micelles- Micelles are aggregates of amphipathic molecules in water, with the nonpolar hydrophobic ends in the interior forming the core and the polar hydrophilic ends at the exterior surface, towards the water surface.

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    Hydrophobic drug can be trapped in the core of such micelles, which effectively protect the drug from the environment. Polar drug will be adsorbed at the micellar surface. In case of amphiphilic drug, the molecules will align themselves in a certain intermediate position [20]. Liposomes- Liposomes are spherical, bilayered vesicles. These are also amphiphilic in nature like micelles. The hydrophilic ends are towards the aqueous side and the hydrophobic ends are oriented away from water. The drug is carried either in the aqueous compartment if hydrophilic or in the lipid bilayers if hydrophobic [15].

    These vesicular carriers can pass through the smallest arterioles and can penetrate endothelium as they are small, flexible and bio-compatible [3]. Niosomes- Niosomes or non-ionic surfactant vesicles are an alternative to liposomes. They overcome the limitations of liposomes such as chemical instability, inconsistent purity of phospholipids and high price. They have a potential use in controlled and targeted drug delivery [15]. Fullerenes- Fullerenes, also called Buckyballs, are composed of carbon.

    They are natural hollow balls and are 1nm in diameter. In fullerenes, active pharmacophores can be coupled to the surface in three-dimensional arrangement to target the compounds to biological sites, to confine atoms within the fullerene cage, and to connect fullerene derivatives to targeting agents [21]. They have many biological actions on the human body and they showed great promise for treatment of various diseases. Targeted or controlled delivery of these macromolecules using nanomaterials, like nanoparticles and dendrimers, is an emerging field called nanobiopharmaceutics.

    Such products are called nanobiopharmaceuticals [22]. These nanobiopharmaceuticals can be formulated as receptor-specific and can be more resistant to unspecific degradation. They can also deliver the peptide in encapsulated form to delay their degradation. Thus, they have an upperhand to conventional peptide delivery systems [14]. Nanoparticulate drug delivery systems have shown potential for enhanced absorption of therapeutic drugs, improved bioavailability, reduced side effects, and sustained intra-ocular drug levels, and thus can be used in the future for the treatment of eye diseases [14].

    These can be overcome by nano drug delivery systems, which work by stabilizing and protecting the release of the drug in bronchi and make pulmonary therapy effective [14].