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Shorter wavelengths are capable of resolving details to a greater degree than are the longer wavelengths.

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There are several equations that have been derived to express the relationship between numerical aperture, wavelength, and resolution: Notice that equation 1 and 2 differ by the multiplication factor, which is 0. These equations are based upon a number of factors including a variety of theoretical calculations made by optical physicists to account for the behavior of objectives and condensers, and should not be considered an absolute value of any one general physical law. In some instances, such as confocal and fluorescence microscopy, the resolution may actually exceed the limits placed by any one of these three equations.

Other factors, such as low specimen contrast and improper illumination may serve to lower resolution and, more often than not, the real-world maximum value of r about 0. The following table Table 1 provides a list resolution r and numerical aperture NA values by objective magnification and correction.

When the microscope is in perfect alignment and has the objectives appropriately matched with the substage condenser, then we can substitute the numerical aperture of the objective into equations 1 and 2 , with the added result that equation 3 reduces to equation 2. An important fact to note is that magnification does not appear as a factor in any of these equations, because only numerical aperture and wavelength of the illuminating light determine specimen resolution.


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As we have mentioned and can be seen in the equations the wavelength of light is an important factor in the resolution of a microscope. Shorter wavelengths yield higher resolution lower values for r and visa versa. The greatest resolving power in optical microscopy is realized with near-ultraviolet light, the shortest effective imaging wavelength. Despite the objections of his wife, Michael decides to make one last attempt to save his friend from drug addiction.

Michael promises to return after one week and follows the map to a run-down house, where he hears Chris ranting and shooting a pistol. Michael cautiously approaches the house and announces himself.

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Chris reacts positively and invites him in, ranting about various subjects. Michael tries to convince Chris to go to rehab, but Chris refuses. Michael uses a stun gun to stun Chris and handcuffs him to a pipe. When Michael returns from a shopping trip, he sees Chris trying to cut through his handcuff with a toothbrush. In the morning, Michael uses the stun gun again to stave off an attempted attack by Chris.

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Later, Micah and Billy show up and tell Michael that Chris is holding their drugs, which they want back. Chris warns Michael not to mess with them, as they have become more violent and unpredictable since Michael last knew them. Michael takes a walk and meets members of a UFO religion , then returns to the house and finds a set of strange photographs. Micah and Billy return, but they are scared off by Charles, a tribal security guard who tells Michael that Chris is squatting on reservation land.

Michael bribes him to allow them to stay there for five days. Michael then finds creepy film footage, and Chris tells Michael that he never sent any emails or videos.

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Michael finds a book full of creepy campfire stories on their doorstep, and, when he returns the book to the library, finds film slides. Confused by the items that seem purposefully left for them to find, Michael suggests that it's a prank by Billy and Micah, but Chris dismisses the idea as irrelevant.

Michael continues to find conspicuous clues strewn about the area. When he bribes Charles for information, Charles warns him off and tells him that French students used the area for research. Use the below workaround for the following symptoms in your JIRA applications: Issues appears as Strike Through on the Activity Stream but not yet resolved or closed.

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An issue has a resolution for an issue that is not resolved. An issue is closed, however it does not have a resolution.

An issue is closed but it still appears on the Assigned to Me. Description There may be times where the resolution of an issue is incorrectly set and you wish to modify it. Product Jira Platform Server.